ディテクター（検出器）としては目的とする物質の性質に応じて光学的性質（吸光度、屈折率、蛍光等）、電気化学的性質、質量分析法などを利用する装置がある。
ディテクターから出力された、電気信号を記録し、そこからピークを検出、解釈を行う。結果は、感熱紙等に印字される。装置のコントロールをしないのであれば、どのメーカーの物を使用しても問題はないが、通常は、装置のコントロールも同時に行うため、同じメーカーの物を選択する。
, which lets us to examine a wide variety of cell phases with only seven experiments. We begin by adjusting the quantity of acetonitrile inside the cell section to produce the best possible separation within the desired Evaluation time.
By following the following pointers and systematically addressing prospective causes, you may successfully troubleshoot popular HPLC difficulties and assure your analyses are accurate and reputable.
. Example of a standard high-performance liquid chromatograph with insets exhibiting the pumps that shift the mobile section throughout the system and the plumbing accustomed to inject the sample in to the cell stage.
. The working pump and the equilibrating pump Every Use a piston whose forwards and backwards motion maintains a relentless flow rate of up to numerous mL/min and supplies the high output force necessary to press the mobile phase in the chromatographic column.
各種の高速液体クロマトグラフィーの項目にある違いは、カラムの違いである事が多いため、装置はそのままでカラムの変更で行える場合が有る。ただし、誤って不適当な溶媒を通すとカラムを破損することがあるため、切り替えを行う際には注意が必要である。
The operating stress in an HPLC is sufficiently high that we are unable to inject the sample in the cell section by inserting a syringe via a septum, as is achievable in gasoline chromatography. In its place, we inject the sample employing a loop injector
The concentration of caffeine in beverages is set by a reversed-stage HPLC separation employing a cellular section of 20% acetonitrile and 80% h2o, and using a nonpolar C8 column. Success for your series of 10-μL injections of caffeine benchmarks are in the next desk.
Enhance or lower the ionization condition of analytes, affecting their affinity for your stationary stage.
- 분석물의 분리여부는 고정상(컬럼)과 이동상의 조합에 의해 결정합니다.(실제 시료 측정에서는 시료 중에 분석물 이외의 오염물질에 존재하는 경우가 많아 분석자는 그 시료의 측정에 최적인 분석 조건의 검토가 필요합니다.
, as an example, demonstrates retention situations for 4 weak acids website in two cell phases with almost similar values for (P^ primary ). Even though the buy of elution is identical for both of those cell phases, Every solute’s retention time is impacted in different ways by the choice of natural solvent.
A reversed-phase HPLC separation is completed using a cellular section of 60% v/v h2o and 40% more info v/v methanol. What's the cell phase’s polarity index?
Although each method is exclusive, the next description in the dedication of fluoxetine in serum provides an instructive illustration of a normal method. The description in this article relies on Smyth, W. F. Analytical Chemistry of Elaborate Matricies